PANC-1 (ATCC® CRL-1469)

Organism: Homo sapiens, human  /  Tissue: pancreas/duct  /  Disease: epithelioid carcinoma

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Organism Homo sapiens, human
Tissue pancreas/duct
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease epithelioid carcinoma
Age 56 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype Chromosome studies indicate a modal number of 63 with 3 distinct marker chromosomes and a small ring chromosome. This is a hypertriploid human cell line. The modal chromosome number was 61, occurring in 32% of cells., However, cells with 63 chromosomes also occurred at a high frequency (22%). The rate of cells with higher ploidies was 8.5%.
Images CRL-1469 Micrograph
Clinical Data
56 years
Caucasian
male
Comments
Growth is inhibited by 1 unit/mL L-asparaginase.
The cells will grow in soft agar.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11,13
D13S317: 11
D7S820: 8,10
D16S539: 11
vWA: 15
THO1: 7,8
Amelogenin: X
TPOX: 8,11
CSF1PO: 10,12
Isoenzymes
G6PD, B
Population Doubling Time 52 hrs
Name of Depositor M Lieber
References

Lieber M, et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870

Wu MC, et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Lieber M, et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870

Wu MC, et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903