PANC-1 (ATCC® CRL-1469)

Organism: Homo sapiens, human  /  Tissue: pancreas/duct  /  Disease: epithelioid carcinoma

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Organism Homo sapiens, human
Tissue pancreas/duct
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease epithelioid carcinoma
Age 56 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype Chromosome studies indicate a modal number of 63 with 3 distinct marker chromosomes and a small ring chromosome. This is a hypertriploid human cell line. The modal chromosome number was 61, occurring in 32% of cells., However, cells with 63 chromosomes also occurred at a high frequency (22%). The rate of cells with higher ploidies was 8.5%.
Images CRL-1469 Micrograph
Clinical Data
56 years
Caucasian
male
Comments
Growth is inhibited by 1 unit/mL L-asparaginase.
The cells will grow in soft agar.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11,13
D13S317: 11
D7S820: 8,10
D16S539: 11
vWA: 15
THO1: 7,8
Amelogenin: X
TPOX: 8,11
CSF1PO: 10,12
Isoenzymes
G6PD, B
Population Doubling Time 52 hrs
Name of Depositor M Lieber
References

Lieber M, et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870

Wu MC, et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Lieber M, et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870

Wu MC, et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903