BT (ATCC® CRL-1390)

Organism: Bos taurus, cow  /  Tissue: turbinate  / 

Organism Bos taurus, cow
Tissue turbinate
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Age newborn
Strain Holstein
Storage Conditions liquid nitrogen vapor phase
Comments
This line has been tested, and found negative for, Bovine viral diarrhea virus (BVDV). The cells should not be grown in medium containing serum of bovine origin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor AW McClurkin
References

McClurkin AW, et al. Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus. Arch. Virusforsch. 45: 285-289, 1974. PubMed: 4472511

Bermudez-Cruz R, et al. Characterization of an abundant, unique 1.7-kilobase bovine herpesvirus 4 (BHV-4) late RNA and mapping of a BHV-4 IE2 transactivator-binding site in its promoter-regulatory region. J. Virol. 71: 527-538, 1997. PubMed: 8985381

Basic Documentation
References

McClurkin AW, et al. Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus. Arch. Virusforsch. 45: 285-289, 1974. PubMed: 4472511

Bermudez-Cruz R, et al. Characterization of an abundant, unique 1.7-kilobase bovine herpesvirus 4 (BHV-4) late RNA and mapping of a BHV-4 IE2 transactivator-binding site in its promoter-regulatory region. J. Virol. 71: 527-538, 1997. PubMed: 8985381