RK3E (ATCC® CRL-1895™)

Organism: Rattus norvegicus, rat  /  Cell Type: E1A immortalized

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Rattus norvegicus, rat
Cell Type E1A immortalized
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 7 days
Strain Fischer
Applications
transfection host
Derivation
The RK3E cell line was derived by immortalizing rat kidney cells with the E1A oncogene.
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
The RK3E cell line was derived by immortalizing rat kidney cells with the E1A oncogene.
The cells can be transformed with the RAS and GLI oncogenes.
The cells have an essentially normal karyotype with the exception of one 5q.
Transformation with RAS or GLI renders the cells tumorigenic.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove old medium, and add fresh 0.25% trypsin, 0.03% EDTA solution. Let sit 2 minutes, remove trypsin and allow the flask to incubate at 37C for 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor K Kinzler, B Vogelstein, J Ruppert
References

Kinzler KW, et al. Identification of an amplified, highly expressed gene in a human glioma. Science 236: 70-73, 1987. PubMed: 3563490

Kinzler KW, Vogelstein B. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Mol. Cell. Biol. 10: 634-642, 1990. PubMed: 2105456

Kinzler KW, et al. The GLI gene is a member of the Kruppel family of zinc finger proteins. Nature 332: 371-374, 1988. PubMed: 2832761

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Kinzler KW, et al. Identification of an amplified, highly expressed gene in a human glioma. Science 236: 70-73, 1987. PubMed: 3563490

Kinzler KW, Vogelstein B. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Mol. Cell. Biol. 10: 634-642, 1990. PubMed: 2105456

Kinzler KW, et al. The GLI gene is a member of the Kruppel family of zinc finger proteins. Nature 332: 371-374, 1988. PubMed: 2832761