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STAT1 Knockout

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STAT1 Knockout models

Customized for your vaccine research

Your own cell lines can slow virus production and limit your ability to get your products to market quickly. Intracellular signaling molecules such as STAT1 are activated in response to viral infection, resulting in arrested production of viral particles – an undesirable bottleneck in vaccine production. Using CRISPR/Cas9 gene editing technology, we are able to disrupt the downstream signaling of this immunomodulator by knocking out STAT1 protein expression in cells commonly used for viral production. These cells produce clinical viruses at titers 10-fold higher than the corresponding parental cell line, potentially reducing time and costs associated with virus and viral vaccine production. Our expertise with CRISPR editing technology and dual CRISPR licenses make us the ideal partner for your project. Our project managers will work with you from conception to production of your STAT1 deficient cell lines.


Data showing dengue viral titer in vero cells

Figure 1. Dengue II New Guinea C virus titer comparison by TCID50 measurement. Vero parental cells and Vero.STAT1 KO cells were seeded and infected with Dengue II New Guinea C virus. Viral supernatants were harvested at day 7 post infection, and then titered by further infecting WT Vero cells in the indicated serial dilutions. The 50% tissue culture infective dose (TCID50) were calculated.

 

 

Data showing Influenza A produced in STAT1 KO MDCK cells at 48 hours postinfection

Figure 2: Influenza A (H1N1; ATCC® VR-1736™) produced in STAT1 KO MDCK cells. Cells were infected with Influenza A at an MOI of 0.01 for 48 hours, then calculated TCID50 of Influenza A viral supernatants produced in MDCK.STAT1KO cells at 48 hours post infection.

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Custom Services Request

Using CRISPR editing technology our experts will create customized STAT1 KO models that meet your virus production needs.




















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CRISPR Editing

ATCC offers custom genome editing services using CRISPR/Cas9 technology that span from project design to cell line development, and we will work with you to develop a cell line that best meets your research needs.

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Cell Engineering

Cell engineering involves physical and engineering principles to control cell behavior. At ATCC, we utilize a number of cell engineering processes including hTERT immortalization and CRISPR/Cas9 gene editing for site-specific genome engineering of cell lines.

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Assay-ready cells

Assay-ready cells give you the flexibility to perform your experiments when you’re ready, without the time and manpower commitments of cell cultivation, maintenance, or counting. ATCC offers expert capabilities for the preparation and characterization of assay-ready cells, either from authenticated ATCC cell lines or your own proprietary cell lines.

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