Utilizing the Scaffolding Ability of Ultra-Long Oxford Nanopore Reads to Assemble Reference-Grade Genomes Originating from Authenticated ATCC Materials

Concentric circles with purple, orange and yellow markers for DNA sequencing.

Nanopore Community Conference

New York City, New York, USA

December 05, 2019


The advancement and accessibility of next-generation sequencing (NGS) technologies have rapidly transformed microbiological research by providing the ability to analyze and profile microbial communities via metagenomics analyses. These sequencing-based applications have relied on the availability of fully assembled reference genomes for bioinformatics analyses, particularly for variant calling in diagnostic and clinical microbiology. However, despite the availability of existing genome sequences in public databases, the quality, completeness, authenticity, accuracy, and traceability of genomic data are inadequate; the lack of standards for genome quality exacerbates these underlying problems. To address this, ATCC has implemented a robust NGS and genome assembly workflow to advance authentication of bacterial strains in the ATCC collection. Our workflow is accompanied by rigorous quality control methods and criteria to ensure that the data proceeding to the next step are the highest quality. Only data that pass all quality control criteria are published to the ATCC Genome Portal, an online database of reference-grade bacterial genomes.

Download the poster to discover more about the hybrid assembly approach used to generate reference-quality genomes.

DNA helix made of green and yellow puffy balls.

ATCC Genome Portal

Your research is important, and you need answers fast. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research. With this cloud-based experience, you can easily download the genome assembly FASTA files you need, view annotations in a linear genome browser or download GBK files, and search for genomes by using your own genomic sequence. With high-quality, annotated data, you can find the answers you need in less time—all you need to get started is a free account!

Create your account on the ATCC Genome Portal