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Validation of Differentiable Molecular Barcodes as Reference Spike-in Oligonucleotides for Microbiome Studies

Poster
Abstract graphic of molecules made of orange and blue balls, and clear tubes containing DNA strands.

ASM Microbe

New Orleans, Louisiana, United States

June 01, 2017

Abstract

A shortcoming in microbiome research is the lack of reference standards to control biases introduced by differential DNA extractability, interference with amplification, library preparation, next-generation sequencing platforms, and data analysis. The aim of this research is to develop and test novel molecular barcodes for use as spike-in reference standards in the form of oligonucleotides or recombinants in microbiome studies.

Download the poster to explore the utility of spike-in standards with unique molecular barcodes in microbiome research.

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