Phytophthora colocasiae Raciborski
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
1. To thaw a frozen ampule, place in a 25-30°C water bath, until just thawed (approximately 5min). Immerse the ampule just enough to cover the frozen material. Do not agitate the ampule.
2. Immediately after thawing, aseptically transfer the contents of the vial onto appropriate moist solid or liquid medium. Some strains revive more readily in liquid media such as ATCC 200: YM broth (= Difco 0711 = BD 271120).
3. Incubate cultures at recommended temperature.
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Gallegly ME, Hong C. Phytophthora; identifying species by morphology and DNA fingerprints. APS Press, St. Paul, Minnesota, USA, 2008.