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Depositors
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R Weiss, Massachusetts Institute of Technology
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Applications
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A TetR homolog-based synthetic promoter for gene expression regulation
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Vector
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Total size of construct (kb): 2.955
Vector type: Entry vector
Markers: kanR
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Insert
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Insert size (kb): 0.18
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
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Related Products
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ATCC SB-1099
ATCC SB-1055
ATCC SB-1056
ATCC SB-1059
ATCC SB-1061
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Biosafety Level
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1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
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Comments
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Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
This construct contains a synthetically designed inducible promoter comprising a TetR homolog PhlF operator and a yeast CYC1 minimal promoter.
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References
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Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737
Ron Weiss, personal communication
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