pCM189 (ATCC® 87661)

Applications: YC-type (centromeric) shuttle vectorexpression vector  /  Depositors: E Herrero

Permits and Restrictions

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Designations pCM189
Depositors E Herrero
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host E. coli DH5α
Vector Information
Size (kb): 8.37
Vector: pCM189 (plasmid)
Promoters: Promoter CMV
Marker(s): URA3,ampR
Construct size (kb): 8.37
Features: marker(s): URA3
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: ARS1
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1
centromere: CEN4
YC-type (centromeric) shuttle vector
expression vector
Restriction digests of the clone give the following sizes (kb): BamHI--8.4; PstI--8.4; BamHI/XhoI--7.7, 0.67.
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672

Related Products
Component of: ATCC 87663
Shipped frozen