Aspergillus nidulans (Eidam) Winter (ATCC® 38163)

Alternate State: Emericella nidulans (Eidam) Vuillemin  /  Strain Designations: G00 [ATCC 12996, ATCC 26451, C. Thom 5616.1, CBS 112.46, FGSC A4, Glasgow wild-type, Pontecorvo strain, IMI 38576ii, NRRL 194, SRRC 273, WB 194, Yuill A69, biA-1]  /  Product Format: freeze-dried

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Strain Designations G00 [ATCC 12996, ATCC 26451, C. Thom 5616.1, CBS 112.46, FGSC A4, Glasgow wild-type, Pontecorvo strain, IMI 38576ii, NRRL 194, SRRC 273, WB 194, Yuill A69, biA-1]
Alternate State Emericella nidulans (Eidam) Vuillemin
Application
Produces ferricrocin
Produces triacetylfusigen
Transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Genotype argB
Preceptrol® no
Genome Sequenced Strain

Yes

Ploidy haploid
Comments
Biotin-dependent as standard wild type for most purposes
Genome sequencing strain (Broad Institute, USA; Nagoya University, Japan; Joint Genome Institute, Department of Energy, USA)
Morphology On PDA medium at 25°C after 6 days, mycelium buff to cinnamon brown becoming army green as conidia develop, velutinous. Reverse tan to reddish brown becoming dark reddish brown. Hyphae hyaline with some stained dark red, guttulate. Conidia globose to subglobose, green, roughened, 4.5-6 X 4.5 µm.
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal   transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGCTGCCTCCGGGCGCCCAACCTCCCACCCGTGACTACCTAACACTGTTGCTTCGGCGGGGAGCCCCCCAGGGGCGAGCCGCCGGGGACCACTGAACTTCATGCCTGAGAGTGATGCAGTCTGAGCCTGAATACAAATCAGTCAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAACTGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGCATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCCCGGCTTGTGTGTTGGGTCGTCGTCCCCCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGTGTCCGGTCCTCGAGCGTATGGGGCTTTGTCACCCGCTCGATTAGGGCCGGCCGGGCGCCAGCCGGCGTCTCCAACCTTATTTTTCTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCCCCTTCGGGGTCCGCGTTGTAATTTGCAGAGGATGCTTCGGGTGCGGCCCCTGTCTAAGTGCCCTGGAACGGGCCGTCAGAGAGGGTGAGAATCCCGTCTTGGGCAGGGTGCCCGTGCCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACCGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTCGGCCCCGGGGTTCAGCCAGCACTCGTGCTGGTGTACTTCCCCGGGGGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCCCCAGGAATGTATCGCCCTCCGGGGTTGTCTTATAGCCTGGGGTGCAATGCGGCCAGCCCGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCGTAATGGTCGCAAACGAC


Calmodulin (CAL)

TATTTGTAAGTGCCATTGGTTACTGTTATATCAAAATCGAATTTGTATTGAGAGTATACTAATACATTCCGCACTAAACAGGACAAGGATGGCGATGGTTAGTGCATCTGTCCCCCCAGGCTTGATCGCATTCGCCCAGCATGTCTGCTGTAGCTCTATATAACCGTTTCTGACAAACGGCGACAGGCCAGATTACCACTAAGGAGCTTGGCACTGTCATGCGCTCGCTCGGTCAGAATCCTTCAGAGTCTGAGCTTCAGGACATGATCAACGAAGTTGACGCCGACAACAATGGCACCATTGACTTTCCAGGTACGCGAACTCCCCAATCTACTTCGCACCAGCCTAGAAATGTACTAATGCTAAACAGAGTTCCTTA

Morphology On PDA medium at 25°C after 6 days, mycelium buff to cinnamon brown becoming army green as conidia develop, velutinous. Reverse tan to reddish brown becoming dark reddish brown. Hyphae hyaline with some stained dark red, guttulate. Conidia globose to subglobose, green, roughened, 4.5-6 X 4.5 µm.
Name of Depositor AJ Clutterbuck
Cross References

Nucleotide (GenBank) : HQ026740 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU729132 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : KU933409 calmodulin gene

Nucleotide (GenBank) : AACD00000000 Aspergillus nidulans FGSC A4, whole genome shotgun sequencing project

References

Charlang G, et al. Cellular and extracellular siderophores of Aspergillus nidulans and Penicillium chrysogenum. Mol. Cell. Biol. 1: 94-100, 1981. PubMed: 6242827

Pontecorvo G, et al. The genetics of Aspergillus nidulans. Adv. Genet. 5: 142-238, 1953.

Zonneveld BJ. Inhibitory effect of 2-Deoxyglucose on cell wall alpha-1,3-glucan synthesis and cleistothecium development in Aspergillus nidulans. Dev. Biol. 34: 1-8, 1973. PubMed: 4595497

Dorn GL. A revised map of the eight linkage groups of Aspergillus nidulans. Genetics 56: 619-631, 1967. PubMed: 6061654

Lin WL, et al. Kinetics of cell growth and heterologous glucoamylase production in recombinant Aspergillus nidulans. Biotechnol. Bioeng. 41: 273-279, 1993.

Timberlake WE. Low repetitive DNA content in Aspergillus nidulans. Science 202: 973-975, 1978. PubMed: 362530

Yadwad VB, et al. Effect of culture conditions and induction strategies on production of human interleukin-6 by a recombinant Aspergillus nidulans strain. Mycol. Res. 100: 356-360, 1996.

Galagan JE, et al. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 438: 1105-1115, 2005. PubMed: 16372000

Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262

Kuprys PV, et al. Identification of telomerase RNAs from filamentous fungi reveals conservation with vertebrates and yeasts. PLoS One 8: e58661, 2013. PubMed: 23555591

Qi X, et al. The common ancestral core of vertebrate and fungal telomerase RNAs. Nucleic Acids Res. 41: 450-462, 2013. PubMed: 23093598

Stajich JE, et al. Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus). Proc Natl Acad Sci USA. 107(26): 11889-11894, 2010. PubMed: 20547848

Glenn AE, et al. Comparative genomic and phylogenetic investigation of the xenobiotic metabolizing arylamine N-acetyltransferase enzyme family. FEBS Lett 584: 3158-3164, 2010. PubMed: 20621844

Min IS, et al. Differential expression of citA gene encoding the mitochondrial citrate synthase of Aspergillus nidulans in response to developmental status and carbon sources. J. Microbiol. 48: 188-198, 2010. PubMed: 20437151

Wortman JR, et al. The 2008 update of the Aspergillus nidulans genome annotation: a community effort. Fungal Genet Biol 46: S2-13, 2009. PubMed: 19146970

Apostolaki A, et al. AgtA, the dicarboxylic amino acid transporter of Aspergillus nidulans, is concertedly down-regulated by exquisite sensitivity to nitrogen metabolite repression and ammonium-elicited endocytosis. Eukaryot Cell 8: 339-352, 2009. PubMed: 19168757

Paoletti M, et al. Mating type and the genetic basis of self-fertility in the model fungus Aspergillus nidulans. Curr. Biol. 17: 1384-1389, 2007. PubMed: 17669651

Ganley AR, Kobayashi T. Highly efficient concerted evolution in the ribosomal DNA repeats: total rDNA repeat variation revealed by whole-genome shotgun sequence data. Genome Res. 17: 184-191, 2007. PubMed: 17200233

Flipphi M, et al. Functional analysis of alcS, a gene of the alc cluster in Aspergillus nidulans. Fungal Genet. Biol. 43: 247-260, 2006. PubMed: 16531087

Chang MH, et al. The GanB Galpha-protein negatively regulates asexual sporulation and plays a positive role in conidial germination in Aspergillus nidulans. Genetics 167: 1305-1315, 2004. PubMed: 15280244

Ram AF, et al. The cell wall stress response in Aspergillus niger involves increased expression of the glutamine : fructose-6-phosphate amidotransferase-encoding gene (gfaA) and increased deposition of chitin in the cell wall. Microbiology 150: 3315-3326, 2004. PubMed: 15470111

Clutterbuck AJ. MA TE transposable elements in Aspergillus nidulans: evidence of repeat-induced point mutation. Fungal Genet. Biol. 41: 308-316, 2004. PubMed: 14761791

Dyer PS, Paoletti M, Archer DB. Genomics reveals sexual secrets of Aspergillus. Microbiology 149: 2301-2303, 2003. PubMed: 12949156

Kim H, et al. The veA gene activates sexual development in Aspergillus nidulans.Fungal Genet. Biol. 37: 72-80, 2002. PubMed: 12223191

Jeong HY, et al. The veA gene is necessary for the inducible expression by fructosyl amines of the Aspergillus nidulans faoA gene encoding fructosyl amino acid oxidase (amadoriase, EC 1.5.3). Arch. Microbiol. 178: 344-350, 2002. PubMed: 12375102

Jeong HY, et al. Differential expression of house-keeping genes of Aspergillus nidulans during sexual development. Gene 262: 215-219, 2001. PubMed: 11179686

Vallim MA, Miller KY, Miller BL. Aspergillus SteA (sterile12-like) is a homeodomain-C2/H2-Zn+2 finger transcription factor required for sexual reproduction. Mol. Microbiol. 36: 290-301, 2000. PubMed: 10792717

Hoffmann B, et al. Developmental and metabolic regulation of the phosphoglucomutase-encoding gene, pgmB, of Aspergillus nidulans. Mol. Gen. Genet. 262: 1001-1011, 2000. PubMed: 10660061

Eckert SE, et al. The tryptophan synthase-encoding trpB gene of Aspergillus nidulans is regulated by the cross-pathway control system. Mol. Gen. Genet. 263: 867-876, 2000. PubMed: 10905354

Pascon RC, Miller BL. Morphogenesis in Aspergillus nidulans requires Dopey (DopA), a member of a novel family of leucine zipper-like proteins conserved from yeast to humans. Mol. Microbiol. 36: 1250-1264, 2000. PubMed: 10931277

Jeong HY, et al. The rpl16a gene for ribosomal protein L16A identified from expressed sequence tags is differentially expressed during sexual development of Aspergillus nidulans. Fungal Genet. Biol. 31: 69-78, 2000. PubMed: 11170736

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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