Staphylococcus aureus subsp. aureus bacteriophage 6
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
GENERAL PROCEDURES FOR THE PROPAGATION OF BACTERIOPHAGE
To titrate phage:
To propagate phage (agar-overlay method):
For broth propagation:
Store filtrate at 4°C. Storage at 20°C may cause inactivation of the phage.
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. Report of the subcommittee on phage-typing of Staphylococci of the international committee on nomenclature of bacteria, Moscow, July 1966. Int. J. Syst. Bacteriol. 17: 113-115, 1967.
. . Bull. World Health Organ. 24: 771-784, 1961.
Evins GM, Armfield AY. Effects of freezing on the stability of staphylococcal phages. Am. J. Clin. Pathol. 44: 480-481, 1965. PubMed: 4220988