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Streptomyces aureofaciens Duggar

10762

Type strain.
Product category
Bacteria
Strain designation
NRRL 2209 [5, ATCC 23884, Boots 876, CBS 664.68, ETH 14302, ETH 17381, HUT 6097, IAM 0087, IFO 12594, IFO 12843, IMRU 3550, ISP 5127, NCIB 8234, PSA 40, RIA 1129, RIA 57, W5]
Type strain
Yes
Isolation source
Soil from timothy field
Geographical isolation
United States; Missouri
Applications
Antibiotic production
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Produces bromide peroxidase bromoperoxidase
Produces chlortetracycline aureomycin
Produces tetracyclines from anhydrotetracyclines
Produces bromoperoxidases BPO 1 and BPO 2
Preceptrol
No

Handling information

Medium
Temperature
26°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial according to enclosed instructions. 
  2. Using a single tube of #1877 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
  4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
  5. Incubate the tubes and plate at 26°C for 7 to 14 days.
Handling notes
On #196 plates aerial mycelia are abundant and medium to dark gray.  Vegetative mycelia are dark mustard brown.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Streptomyces aureofaciens Duggar
Depositors
NRRL
Chain of custody
ATCC <-- NRRL <-- Lederle Labs. A-377
Type of isolate
Environmental
Cross references
GenBank L22864 Streptomyces aureofaciens whiB operon, complete sequence.
GenBank M84990 Streptomyces aureofaciens chloroperoxidase CPO-A2 (cpoA2), complete cds.
GenBank AF010134 Streptomyces aureofaciens putative sporulation protein gene, complete cds.
GenBank U01096 Streptomyces aureofaciens bromoperoxidase BPO-A1 (bpoA1) gene, complete cds.
GenBank L11647 Streptomyces aureofaciens glycogen branching enzyme (glgB) gene, complete cds.
GenBank AF007125 Streptomyces aureofaciens elongation factor G (fus) gene, partial cds, and elongation factor Tu (tuf1) gene, complete cds.
GenBank AF096293 Streptomyces aureofaciens sporulation transcription factor (whiH) gene, complete cds; and sigma factor (hrdB) gene, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Miller PA, McCormick JR. Biological conversion of anhydrotetracyclines to tetracyclines. US Patent 3,053,740 dated Sep 11 1962

Duggar BM. Aureomycin and preparation of same. US Patent 2,482,055 dated Sep 13 1949

Ettlinger L, et al. Zur Systematik der Actinomyceten. 4. Eine arteinteilung der Gattung Streptomyces Waksman et Henrici. Arch. Mikrobiol. 31: 326-358, 1958.

Pfeifer O, et al. Molecular cloning and sequencing of a non-haem bromoperoxidase gene from Streptomyces aureofaciens ATCC 10762. J. Gen. Microbiol. 138: 1123-1131, 1992. PubMed: 1527491

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

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