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Microbial Genome Sequencing and Assembly: Existing Challenges and the Need for Authentic Reference Genomes

Poster
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ASM Microbe 2019

San Francisco, California, United States

June 20, 2019

Abstract

The advancement and accessibility of next-generation sequencing (NGS) technologies have rapidly transformed microbiological research by providing the ability to analyze and profile microbial communities via metagenomics analyses. These sequencing-based applications have relied on the availability of fully assembled reference genomes for bioinformatics analyses, particularly for variant calling in diagnostic and clinical microbiology. However, despite the availability of existing genome sequences in public databases, the quality, completeness, authenticity, accuracy, and traceability of genomic data is inadequate; the lack of standards for genome quality exacerbates these underlying problems. To address this, ATCC has implemented a robust NGS and genome assembly workflow to advance authentication of bacterial strains in the ATCC collection.

Download the poster to learn more about the robust NGS and genome assembly workflow used at ATCC.

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ATCC Genome Portal

We have developed a standardized genome sequencing and assembly workflow to provide researchers with the whole-genome sequences of the specific, authenticated materials needed to generate credible data. Each sequence was developed using an optimized methodology that combines the power of highly accurate short reads with the revolutionary scaffolding ability of ultra-long reads. We then took our workflow one step further by accompanying each stage of the process with rigorous quality control analyses that ensure our data are the highest quality possible. Only the data that passes all quality control criteria are published to the ATCC Genome Portal. Visit the portal today to find the high-quality data you need for your research.

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