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External Validation of ATCC Molecular Standards for Herpes Simplex Virus (HSV-1 and HSV-2)

Poster
Broken DNA helix made of purple balls.

CVS 2016

Daytona Beach, Florida, United States

May 16, 2016

Abstract

Herpes simplex virus (HSV-1 and HSV-2) causes a wide range of clinical manifestations that result in lifelong infections. Quantitative PCR (qPCR) assays are routinely used for the detection of HSV-1 and HSV-2 infections in clinical samples. However, the accuracy of a qPCR assay is dependent upon the generation of a standard curve using a positive control with a known genome copy number. Moreover, an independent positive control is required to monitor variations in assay performance for molecular assays. ATCC has developed HSV-1 and HSV-2 quantitative molecular standards for use as controls for the detection and quantification of these viruses from clinical samples.

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