To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org. For Technical questions please contact tech@atcc.org. Thank you.
X

FAQ's

FAQ Home

Find Answers

PC-12 Cells: ATCC® CRL-1721 vs. ATCC® CRL-1721.1
What is the difference between ATCC® CRL-1721 ™ and ATCC® CRL-1721.1 ™?

 PC-12Adh, ATCC® CRL-1721.1 ™ is an adherent variant of PC-12. Its official designation is "PC-12 Adh". These cells exhibit the well-attached adherent phenotype. They were isolated after repeated exposure to Corning's CellBIND flasks (Corning Catalog#3289 through 3293), and exhibit an increased growth rate when compared to the traditional PC-12 cells that grow in suspension (ATCC® CRL-1721 ™, PC-12). ATCC has not tested CRL-1721.1 cells for differentiation potential in response to Nerve Growth Factor (NGF) treatment.

PC-12Adh, ATCC® CRL-1721.1 ™ cells are grown in ATCC-formulated F12K media (ATCC Cat. No. 30-2004) with 2.5% non-heat inactivated FBS and 15% non-heat inactivated horse serum.  When cultured on the CellBIND flasks, these cells exhibit a heterogeneous mixture of small epithelial-shaped cells, some stellate shaped cells, some elongated cells and some rounded but attached cells.

The original PC-12 cells (ATCC® CRL-1721 ™) have never been exposed to Corning's CellBIND flasks and retain a high differentiation potential in response to NGF treatment.

PC-12, ATCC® CRL-1721 ™ cells are grown in ATCC-formulated RPMI-1640 media (ATCC® 30-2001 ™) with 5% non-heat inactivated FBS (ATCC® 30-2020 ™) and 10% heat inactivated horse serum.  The PC-12 cells grow as small, irregularly shaped cells, loosely adherent or as multi-cell aggregates floating in the growth media.  They have a tendency to aggregate and adhere poorly to non-coated surfaces. The clusters may be dispersed by gentle aspiration with a syringe and 22g needle and a 20ml Luer-Lok Tip syringe 4-5 times when initiating or expanding the culture.  Collagen IV is only used when testing for neurite formation. 

ATCC has performed QC tests on seed stocks of CRL-1721 cells for neurite outgrowth in response to NGF treatment. Cells are seeded at 5 x 103 cell/cm2, 2.5 x 103 cells/cm2 and 1.25 x 103 cells/cm2 on Corning® BioCoat™ Collagen IV 6-well plates (Cat No. 354428) and incubated for 3 days to allow for cell attachment.  On day 3, the culture media is replaced with the test medium, consisting of the complete growth media supplemented with a final concentration of 0.2 ug/ml final concentration of NGF.  Neurites begin to form within 3 to 4 days after initial treatment.


Date Created07/17/2012 07:14 PM
Date Updated07/01/2014 07:43 AM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. Passage number vs. population doubling level (PDL)
  4. Converting TCID[50] to plaque forming units (PFU)
  5. U-373 MG (ATCC® HTB-17)