ATCC Media, Sera, and Reagents

Animal Cell Culture Guide ATCC Media, Sera, and Reagents

Table of Contents

Media, Sera, and Reagents

ATCC Media Product List

ATCC® No. Product Name Volume
30-2002
Dulbecco’s Modified Eagle’s Medium (DMEM) 500 mL
SCRR-2010
ES-DMEM 500 mL
30-2006
DMEM:F12 Medium (1:1 Ratio) 500 mL
30-2003
Eagle’s Minimum Essential Medium (EMEM) 500 mL
30-2004
F12K Medium 500 mL
46-X
Hybri-Care Medium, Powder Prepares 1 L
30-2005
Iscove’s Modified Dulbecco’s Medium (IMDM) 500 mL
30-2008
Leibovitz’s L-15 Medium 500 mL
30-2007
McCoy’s 5A Medium, Modified 500 mL
30-2001
RPMI-1640 Medium 500 mL

With the exception of Leibovitz’s L-15 Medium, ATCC media are formulated* for use with 5% CO2 . The amount of sodium bicarbonate in each media ranges from 1.2 g/L to 2.2 g/L (1.5 g/L is the most common concentration).

* See the ATCC website for complete media formulations.


Animal Sera Product List

ATCC® No. Product Name Volume
30-2020 Fetal Bovine Serum 500 mL
30-2021 Fetal Bovine Serum 100 mL
SCRR-30-2020 Fetal Bovine Serum, ES Qualified* 500 mL
30-2030 Calf Bovine Serum 500 mL
30-2031 Calf Bovine Serum 100 mL
30-2040 Horse Serum 500 mL
30-2041 Horse Serum 100 mL

*Qualified for mouse and human embryonic stem cells.


Supplements and Antibiotics Product List

ATCC® No. Product Name Volume
30-2214 L-Glutamine Solution 100 mL
PCS-999-002 Penicillin-Streptomycin-Amphotericin B Solution 1.0 mL
30-2300 Penicillin-Streptomycin Solution 100 mL


Buffers, Stains, and Dissociation Reagents Product List

ATCC® No. Product Name Volume
30-2103 Non-Enzymatic Cell Dissociation Solution 100 mL
30-2104 Soybean Trypsin Inhibitor 20 mL
30-2101 0.25% Trypsin/0.53 mM EDTA Solution 100 mL
PCS-999-003 Trypsin-EDTA for Primary Cells* 100 mL
PCS-999-004 Trypsin Neutralizing Solution 100 mL
30-2200 Dulbecco’s Phosphate Buffered Saline (DPBS)* 500 mL
30-2213 Hank’s Balanced Salt Solution (HBSS)* 500 mL
PCS-999-001 Phenol Red 1.0 mL


Cryopreservation Reagents Product List

ATCC® No. Product Name Volume
4-X Dimethylsulfoxide (DMSO) 5 x 5 mL
30-2600 Serum-Free Cell Freezing Medium 20 mL


Supplements and Antibiotics Product List

ATCC® No. Product Name Volume
PCS-999-027 0.1% Gelatin Solution 100 mL


Cell Proliferation Assays and Mycoplasma Detection

ATCC® No. Product Name
30-1010K ATCC MTT Cell Proliferation Assay
30-1011K XTT Cell Proliferation Assay Kit
30-1012K Universal Mycoplasma Detection Kit