Over the last several years, ATCC has pushed microbial authentication into the 21st century. We have always used a variety of methods to authenticate our microbes but have recently started to systematically complete whole-genome sequencing on items in our microbial collection. Our goal is to provide the research community with authentic, reproducible, and traceable reference genome assemblies derived from physical material. To date, we have sequenced thousands of microbes in our collection and have published 4,500 microbial genomes to the ATCC Genome Portal (AGP).