pPL703 (ATCC® 37219)

Applications: promoter-cloning vectorshuttle vector  /  Depositors: PS Lovett

Designations pPL703
Depositors PS Lovett
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.5000000000000000
Vector: pPL703 (plasmid)
Promoters: Promoter none
Construction: pPL603, M13mp7 polylinker
Construct size (kb): 4.5
Features: insert detection: CAT
marker(s): neoR
promoter: none
replicon: pUB110
promoter-cloning vector
shuttle vector
The PstI site is 144 bp upstream from cat-86.
Cat-86 is not expressed until a promoter-containing fragment is cloned into one of the polylinker sites.
A Bacillus subtilis promoter cloning plasmid vector.
Media ATCC® Medium 1179: VY medium
Growth Conditions
Temperature: 37.0°C

Duvall EJ, et al. Chloramphenicol-inducible gene expression in Bacillus subtilis. Gene 24: 171-177, 1983. PubMed: 6416927

Mongkolsuk S, et al. Restriction fragments that exert promoter activity during postexponential growth of Bacillus subtilis. J. Bacteriol. 155: 1399-1406, 1983. PubMed: 6309749

Shipped freeze-dried