pMH621 (ATCC® 31775)

Applications: expression vectorvector with sequences for protein export  /  Depositors: NCI-Frederick Cancer Research and Development Center, TJ Silhavy, NCI-Frederick Cancer Research and Development Center

Permits and Restrictions

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Designations pMH621
Depositors NCI-Frederick Cancer Research and Development Center, TJ Silhavy, NCI-Frederick Cancer Research and Development Center
Biosafety Level 1
Host
Distribution host: Escherichia coli MH2000
Distribution host: Escherichia coli MH2000
Vector Information
Size (kb): 7.5999999046325680
Vector: pMH621 (plasmid)
Promoters: Promoter ompF
Marker(s):ampR
Construct size (kb): 7.599999904632568
Features: marker(s): ampR
promoter: ompF
replicon: pMB1
terminator: none
enhancer: none
Applications
expression vector
vector with sequences for protein export
Comments
Expression vector permitting export of a cloned product by formation of a fusion protein with the signal peptide and 12 amino-terminal amino acids of mature OmpF.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: CSHL Press; 1982.

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet