108735 (ATCC® 87666)

Organism: Cryptosporidium muris Tyzzer  /  Clone Type: Library  /  Depositors: SJ Upton, NV Khramtsov

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Designations 108735
Species Cryptosporidium muris Tyzzer
Depositors SJ Upton, NV Khramtsov
Tissue: sporozoites/oocysts
DNA: genomic
Digest: Complete
Insert size range: 0.15 - 0.8kb
Coverage: Clones for "complete" coverage: 16000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

DNA was isolated from oocysts of Cryptosporidium muris, 108735 isolate, obtained originally from the feces of a 5 year old female rock hyrax housed at the National Zoological Park Zoo in July of 1993. This was designated as case number 93-1677.
Since 1993, the isolate has been passed continuously at University of Idaho, in juvenile laboratory mice. The library represents DNA obtained from oocytes collected after the 16th passage in mice.

Khramtsov NV, et al. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form hsp70. J. Eukaryot. Microbiol. 42: 416-422, 1995. PubMed: 7620467

Esteban E, Anderson BC. Cryptosporidium muris: prevalence, persistency, and detrimental effect on milk production in a drylot dairy. J. Dairy Sci. 78: 1068-1072, 1995. PubMed: 7622718