lambda93 [GC.93] (ATCC® 95664)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: A Dugaiczyk

Permits and Restrictions

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Designations lambda93 [GC.93]
GenBank Number

L10641

Species Homo sapiens, human
Depositors A Dugaiczyk
Vector
Construct size (kb): 51.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 19.95
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-19950 of the insert correspond to
nucleotides ~6300-26300 of L10641.
Insert lengths(kb): 19.95000076293945
Tissue: fetal liver (ATCC 37333)
Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]
Insert Size (kb) 19.950
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11.0, 5.0, 3.7, 2.6, 2.55, 1.9, 1.8, 0.95, 0.7; HindIII-->23, 5.9; BamHI-->23, 7.3, 5.7, 4 (doublet), 1.6;
BglII--5.5, 5.1, 4.9 (doublet), 3.5, 3.1, 2.75, 2.5, 2.15, 2.0, 1.8 (doublet), 1.7, 1.55 (doublet), 1.4, 0.65; SstI-->23, 9.0, 4.1, 3.9, 2.8, 1.9.
ATCC 95662/95663 and 95664/95665 overlap by 5. kb and share EcoRI fragments of the following sizes (kb): 0.7, 1.7.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--3.3, 4.0, 5.7, 9.4, 12.0, 12.6, 14.4, 14.55, 16.45, 19; HindIII--1.6; SacI--9, 11.8, 15.9.
Begins in intron 1 and continues into intron 6.
ATCC 95664/95665 and 95666/95667 overlap by 6.45 kb and share EcoRI fragments of the following sizes (kb): 0.15, 1.9, 2.5.
Contains L1 (Kpn) and Alu repeats.
References

Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650