lambda93 [GC.93] (ATCC® 95664)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: A Dugaiczyk

Permits and Restrictions

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Designations lambda93 [GC.93]
GenBank Number

L10641

Species Homo sapiens, human
Depositors A Dugaiczyk
Vector
Construct size (kb): 51.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 19.95
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-19950 of the insert correspond to
nucleotides ~6300-26300 of L10641.
Insert lengths(kb): 19.95000076293945
Tissue: fetal liver (ATCC 37333)
Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]
Insert Size (kb) 19.950
Biosafety Level 1
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11.0, 5.0, 3.7, 2.6, 2.55, 1.9, 1.8, 0.95, 0.7; HindIII-->23, 5.9; BamHI-->23, 7.3, 5.7, 4 (doublet), 1.6;
BglII--5.5, 5.1, 4.9 (doublet), 3.5, 3.1, 2.75, 2.5, 2.15, 2.0, 1.8 (doublet), 1.7, 1.55 (doublet), 1.4, 0.65; SstI-->23, 9.0, 4.1, 3.9, 2.8, 1.9.
ATCC 95662/95663 and 95664/95665 overlap by 5. kb and share EcoRI fragments of the following sizes (kb): 0.7, 1.7.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--3.3, 4.0, 5.7, 9.4, 12.0, 12.6, 14.4, 14.55, 16.45, 19; HindIII--1.6; SacI--9, 11.8, 15.9.
Begins in intron 1 and continues into intron 6.
ATCC 95664/95665 and 95666/95667 overlap by 6.45 kb and share EcoRI fragments of the following sizes (kb): 0.15, 1.9, 2.5.
Contains L1 (Kpn) and Alu repeats.
References

Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650

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