Simian virus 40 (ATCC® VRMC-6)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

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Designations pUCSV-VA45-54-2Ea [molecular clone of Simian virus 40, SV-40]
GenBank Number


Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
contains sequence
Construct size (kb): 7.949999809265137
Insert lengths(kb): 5.263999938964844
Tissue: uninoculated grivet monkey cells
Gene product: [SV-VA45-54-2Ea]
Insert Size (kb) 5.264
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Insert contains following restriction sites (from 5'end): BamHI-0.771,7.179; EcoRI-2.686,5.264; KpnI-3.786,4.164; SalI-7.950; SfiI-7.950; XhoI-uncut. Plasmid size: 7950bp (2686bp pUC-19vector+ 5264bp SV40-VA45-54-2 insert).
This variant of SV-40-VA45-54 has an non-archetypal viral regulatory region and was verified by lipofection of EcoRI-linearized plasmid pUCSV-VA45-54-2Ea DNA into ATCC CV-1 cells by the contributor, followed by DNA sequence analysis of the virus stock.
Classification Polyomaviridae, Polyomavirus

Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955