Recommended Host
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Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™)
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Growth Conditions
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Temperature: 28°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80% confluent. Remove medium and then wash monolayer with PBS or serum free medium prior to inoculation. Inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb for 1 hour at 28°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 4-6 days at 28°C in a humidified 5% CO2 atmosphere, until CPE is progressed throughout monolayer.
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