Trichoderma virens (Miller et al.) von Arx, anamorph (ATCC^® MYA-4894^™)

Alternate State: Hypocrea virens Chaverri et al., teleomorph / Strain Designations: FGSC 10586 [Tv29-8, Gv29-8] / Product Format: frozen

Trichoderma virens (Miller et al.) von Arx, anamorph ATCC^® MYA-4894^™

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Permits and Restrictions	View Permits
Strain Designations	FGSC 10586 [Tv29-8, Gv29-8]
Alternate State	Hypocrea virens Chaverri et al., teleomorph
Biosafety Level	1
Product Format	frozen
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C
Type Strain	no

Preceptrol®	no
Genome Sequenced Strain	Yes
Comments	mycoparasite Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA)
Morphology	On PDA medium at 25°C after 9 days, colonies dark green covered in a dense layer of white cottony mycelium. Reverse tan to yellow brown. Hyphae hyaline, guttulate. Conidiophores branched. Phialides in whorls of 3-4, ovoid, 10.5-12 X 4.5 µm. Conidia dark green in mass, pale green singly, globose to subglobose, 3-4.5 X 3-4.5 µm.

Medium	ATCC^® Medium 200: YM agar or YM broth ATCC^® Medium 324: Malt extract agar ATCC^® Medium 336: Potato dextrose agar (PDA)
Growth Conditions	Temperature: 20°C to 25°C Atmosphere: Typical Aerobic

Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence; GenBank number JX174053: AGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGCGGGATCTCTGCCCCGGGTGCGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCAAAACTCTTATTGTATACCCCCTCGCGGGTTTTTTACTATCTGAGCCATCTCGGCGCCCCTCGTGGGCGTTTCGAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTTTACGGGGCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAGCGCGGCGCGTCCACAGCCGTTAAACACCCCAAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAG D1D2 region of the 28S ribosomal RNA gene; GenBank number JX174042: TTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCTCGGGGTCCGAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTCTGGCTGGCCACCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACCTTGAAAAGAGGGTTAAATAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGTGACCAGACTTGGGCGCGGCGGATCATCCGGGGTTCTCTCCGGTGCACTTCGCCGCGTCTAGGCCAGCATCAGTTCGTCGCGGGGGAAAAAGGCTTCGGGAACGTGGCTCCTCCGGGAGTGTTATAGCCCGTTGCATAATACCCTGCGGTGGACTGAGGACCGCGCATCTGCAAGGATGCTGGCGTAATGGTCACCAGCGA
Morphology	On PDA medium at 25°C after 9 days, colonies dark green covered in a dense layer of white cottony mycelium. Reverse tan to yellow brown. Hyphae hyaline, guttulate. Conidiophores branched. Phialides in whorls of 3-4, ovoid, 10.5-12 X 4.5 µm. Conidia dark green in mass, pale green singly, globose to subglobose, 3-4.5 X 3-4.5 µm.

Sequenced Data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence; GenBank number JX174053:

AGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGCGGGATCTCTGCCCCGGGTGCGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCAAAACTCTTATTGTATACCCCCTCGCGGGTTTTTTACTATCTGAGCCATCTCGGCGCCCCTCGTGGGCGTTTCGAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTTTACGGGGCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAGCGCGGCGCGTCCACAGCCGTTAAACACCCCAAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAG

D1D2 region of the 28S ribosomal RNA gene; GenBank number JX174042:

TTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCTCGGGGTCCGAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTCTGGCTGGCCACCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACCTTGAAAAGAGGGTTAAATAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGTGACCAGACTTGGGCGCGGCGGATCATCCGGGGTTCTCTCCGGTGCACTTCGCCGCGTCTAGGCCAGCATCAGTTCGTCGCGGGGGAAAAAGGCTTCGGGAACGTGGCTCCTCCGGGAGTGTTATAGCCCGTTGCATAATACCCTGCGGTGGACTGAGGACCGCGCATCTGCAAGGATGCTGGCGTAATGGTCACCAGCGA

Morphology

On PDA medium at 25°C after 9 days, colonies dark green covered in a dense layer of white cottony mycelium. Reverse tan to yellow brown. Hyphae hyaline, guttulate. Conidiophores branched. Phialides in whorls of 3-4, ovoid, 10.5-12 X 4.5 µm. Conidia dark green in mass, pale green singly, globose to subglobose, 3-4.5 X 3-4.5 µm.

Name of Depositor	FGSC
Special Collection	ATCC
Chain of Custody	ATCC <-- FGSC
Cross References	Nucleotide (GenBank) : JX174053 ITS including 5.8S rRNA gene Nucleotide (GenBank) : JX174042 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : ABDF00000000 Trichoderma virens Gv29-8, whole genome shotgun sequencing project
References	Kubicek CP, et al. Comparative genome sequence analysis underscores mycoparasitism as the ancestral life style of Trichoderma. Genome Biol. 12: R40, 2011. PubMed: 21501500

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS

Trichoderma virens (Miller et al.) von Arx, anamorph (ATCC® MYA-4894™)

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Trichoderma virens (Miller et al.) von Arx, anamorph (ATCC^® MYA-4894^™)