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Escherichia coli (Migula) Castellani and Chalmers

BAA-2778 ™

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This strain is part of the Global Priority Superbugs collection. It is an extensively characterized antimicrobial-resistant clinical isolate with validated genotypic and phenotypic activity against a variety of drug classes. This strain is provided with geographic source information; an NGS-­assembled whole genome sequence, including annotated antibiotic resistance and rRNA genes; and an expanded level of susceptibility data with MIC test results, making it an essential tool for all stages of the discovery and development process for novel antimicrobials and therapeutics, molecular-­based detection assays, and updated sterility protocols.

This product has been removed from the ATCC catalog and will be deposited with the Biodefense and Emerging Infectious Resources (BEIR) collection.

Product category
Bacteria
Product type
Drug-resistant bacterium
Classification
Enterobacteriaceae, Escherichia
Strain designation
1123005
Type strain
No
Genome sequenced strain
Yes
Isolation source
Urine
Geographical isolation
Nigeria
Applications
Antimicrobial resistance research
Bioinformatics
Drug development
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

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Read more about safety information about this product

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

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Detailed product information

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General

Specific applications
Drug discovery and development
Development of molecular-based detection assays
Development of updated sterility protocols
Reports
Upon your purchase of this product, you will receive a report containing MIC values, a susceptibility profile, and the DNA sequence information for predicted annotated antibiotic resistance genes and 16S rRNA genes. This report will be provided in multiple formats (.pdf, .txt, .xls, .zip).
Preceptrol
No

Characteristics

Gram stain
Negative
Annotated genes
AAC6-Ib-cr
AAC3-IIA
aadA5
acrA
acrB
acrD
acrE
acrF
APH(3")-Ib
APH(6)-Id
baeR
CMY-2
CMY-47
CTX-M-15
emrB
macA
macB
marA
mdtB
mdtC
mdtF
msbA
OXA-1
TEM-1
tet(A)
tolC
Susceptibility profile
Resistant
Aztreonam
Cefepime
Ceftazidime
Ceftriaxone
Ciprofloxacin
Levofloxacin
Piperacillin-tazobactam
Tetracycline
Trimethoprim-sulfamethoxazole

Intermediate
Amoxicillin-clavulanate

Susceptible
Amikacin
Doripenem
Fosfomycin
Imipenem
Meropenem
Comments
This strain is part of the Global Priority Superbugs collection. It is an extensively characterized antimicrobial-resistant clinical isolate with validated genotypic and phenotypic activity against a variety of drug classes. This strain is provided with geographic source information; an NGS-assembled whole genome sequence, including annotated antibiotic resistance and rRNA genes; and an expanded level of susceptibility data with MIC test results.

Minimum inhibitory concentration (MIC) ranges for resistant, intermediate, and susceptible are based on criteria within the Clinical and Laboratory Standards Institute (CLSI) Performance Standards for Antimicrobial Susceptibility Testing, 26th Edition.

Predicted antimicrobial resistance gene sequence data are provided for informational purposes only and are based on open reading frames (ORFs) identified and annotated from contigs produced during next-generation sequencing (NGS). ATCC does not warrant that the entire predicted antimicrobial resistance gene is present within the sequence prepared for lot-specific reports. Likewise, the information provided merely indicates that some portion of the sequence (whole or in part) is present based on analytical base pair alignment with known gene sequences.

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Aseptically transfer the entire contents to a 5-6 mL tube of #18 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #18 plate and/or #18 agar slant.
  4. Incubate at 37°C for 18-24 hours.
Handling notes
Predicted antimicrobial resistance gene sequence data are provided for informational purposes only and are based on open reading frames (ORFs) identified and annotated from contigs produced during NextGen Sequencing (NGS). ATCC does not warrant that the entire predicted antimicrobial resistance gene is present within the sequence prepared for lot-specific reports. Likewise, the information provided merely indicates that some portion of the sequence (whole or in part) is present based on analytical base pair alignment with known gene sequences.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Escherichia coli
Depositors
International Health Management Associates (IHMA), Inc.
Chain of custody
ATCC <-- International Health Management Associates (IHMA), Inc.
Year of origin
2014
Patient age
46 years
Patient gender
Female
Special collection
Global Priority Superbugs

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Material Transfer Agreement Addendum for Global Priority Superbug Products

For every order of this item, you must provide a signed Global Priority Superbugs Addendum. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.

Email the signed addendum to [email protected] with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

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References

Curated Citations

Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing, 26th Edition. Wayne, PA. M100S, 2016.

Related Resources

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