Size (kb): 4.9000000953674320
Vector: pKKM13(+) (phagemid)
Promoters: Promoter for expression tac
Construct size (kb): 4.900000095367432
Features: marker(s): ampR
promoter for expression: tac
transcription terminator: T1
transcription terminator: T2
vector permitting production of single-stranded DNA
vector useful for site-directed mutagenesis
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BamHI--4.6, 0.2; XbaI--4.8.
Expression vector useful for mutagenesis of cloned inserts.
Single stranded DNA obtained after superinfection with a suitable M13 helper phage gives the sense strand with respect to the tac promoter.
Nock S, et al. One-vector system for mutagenesis and over-expression of cloned genes. BioTechniques 18: 608, 1995. PubMed: 7598889
Roland Kreutzer, personal communication