pLEX5B [WM2284] (ATCC® 87201)

Applications: cloning vector  /  Depositors: W Messer

Designations pLEX5B [WM2284]
Depositors W Messer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.6170001029968260
Vector: pLEX5B (plasmid)
Construction: pT7-7, pNM480
Construct size (kb): 2.617000102996826
Features: marker(s): ampR
replicon: pMB1
MCS: XmnI...HindIII
ribosome-binding site: Shine-Dalgarno
transcription terminator: rrnB T1T2
cloning vector
Restriction digests of the clone give the following sizes (kb): EcoRI--2.6; HindIII--2.6; XmnI--2.6.
Expression vector with efficient prokaryotic expression signals upstream of the XmnI site and rrnB terminators downstream.
Expression requires fusion of a 5'TG of the insert with the A nucleotide of the XmnI site of the vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried