pBSL141 (ATCC® 87146)

Applications: contains easily purifiable cassette(s) for construction gentamicin acetyltransferase  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL141
Depositors MF Alexeyev
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.8859999179840090
Vector: pBSL141 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Construct size (kb): 3.885999917984009
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pBM1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aacC1 (gtmR)
contains easily purifiable cassette(s) for construction gentamicin acetyltransferase
Restriction digests of the clone give the following sizes (kb): MluI--3.1, 0.8; BamHI--2.9, 1.0; NotI--2.9, 1.0.
Problems have been reported when using DH5alpha as a host for multicopy plasmids bearing Gm and Tc, but not when TG1 is used.
Plasmid useful for vector construction. Contains a gentamicin acetyltransferase (aacC1, gtmR) cassette flanked by multiple cloning sites duplicated in tandem orientation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Mikhail F Alexeyev, personal communication

Shipped frozen
Shipping Information

 Frozen glycerol stock of E. coli containing the phagemid