pBSL141 (ATCC® 87146)

Applications: contains easily purifiable cassette(s) for construction gentamicin acetyltransferase  /  Depositors: MF Alexeyev

Designations pBSL141
Permits and Restrictions

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Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 3.8859999179840090
Vector: pBSL141 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Construct size (kb): 3.885999917984009
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pBM1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aacC1 (gtmR)
contains easily purifiable cassette(s) for construction gentamicin acetyltransferase
Restriction digests of the clone give the following sizes (kb): MluI--3.1, 0.8; BamHI--2.9, 1.0; NotI--2.9, 1.0.
Problems have been reported when using DH5alpha as a host for multicopy plasmids bearing Gm and Tc, but not when TG1 is used.
Plasmid useful for vector construction. Contains a gentamicin acetyltransferase (aacC1, gtmR) cassette flanked by multiple cloning sites duplicated in tandem orientation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Mikhail F Alexeyev, personal communication

Shipped frozen
Shipping Information

 Frozen glycerol stock of E. coli containing the phagemid