pYAC4 (ATCC® 67379)

Applications: YL-type (YAC) shuttle vectorShuttle vectorVector for constructing genomic libraries  /  Depositors: MV Olson

Designations pYAC4
Permits and Restrictions

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Depositors MV Olson
U.S. Patent
Disclosure FPAT
Biosafety Level 1
U.S. Patent
Vector Information
Size (kb): 11.4540004730224600
Vector: pYAC4 (YAC)
Marker(s): TRP1,URA3,ampR,HIS3,SUP4
Construct size (kb): 11.454
Features: marker(s): HIS3
marker(s): SUP4
marker(s): TRP1
marker(s): URA3
marker(s): ampR
other: telomere
replicon: ARS1
replicon: pMB1
centromere: CEN4
YL-type (YAC) shuttle vector
Shuttle vector
Vector for constructing genomic libraries
Yeast artificial chromosome (YAC) vector for cloning EcoRI fragments
A 0.7 kb BamHI-XhoI fragment contains two copies of the Tetrahymena thermophila macronuclear telomere
This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825

Wells RA, et al. Telomere-related sequences at interstitial sites in the human genome. Genomics 8: 699-704, 1990. PubMed: 2276741

Burke DT, Olson MV. Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 194: 251-270, 1991. PubMed: 2005791

Kuhn RM, Ludwig RA. Complete sequence of the yeast artificial chromosome cloning vector pYAC4. Gene 141: 125-127, 1994. PubMed: 8163163

Shipped frozen