Thauera butanivorans Dubbels et al. ex Takahashi et al.

1. Open vial according to enclosed instructions.                            
2. Using a single tube of #1581 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet. 
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a #1581 or #44 slant, and/or a #1581 or #44 plate. 
5. Place tubes and plate in an anaerobe jar and pull the vacuum to –3.6” Hg (0.12 Atm.).  Add n-butane to –1.5” Hg (0.07 Atm).  Add CO₂ to fill (0.05 Atm) with 88% air.
6. Incubate at 30°C for 96 hours.

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