pSV2-hph (ATCC® 37647)

Applications: co-transfection marker aminocyclitol phoshotransferase hygromycin B phosphotransferaseexpression vectorshuttle vector  /  Depositors: P Berg, M Dieckmann

Permits and Restrictions

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Designations pSV2-hph
Depositors P Berg, M Dieckmann
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5000000000000000
Vector: pSV2-hph (plasmid)
Promoters: Promoter SV40 early
Construct size (kb): 5.5
Features: marker(s): ampR, hygR
promoter: SV40 early
replicon: pMB1
terminator: SV40
enhancer: SV40 early
co-transfection marker aminocyclitol phoshotransferase hygromycin B phosphotransferase
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): HindIII/BglII--3.87, 1.34, 0.69; PvuII/PstI--3.66, 1.84; PvuII--uncut.
An Escherichia coli/mammalian cell shuttle vector.
Consists of the SV40 promoter (SV nt 270-5171), a HindIII linker, the hph gene (1347 bp), SV40 splice site (SV nt 4710-4100), SV40 polyadenylation site (SV nt 4362-2067),
pML (inserted at the BamHI site with HindIII-EcoRI-amp upstream of the SV40 promoter).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gritz L, Davies J. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179-188, 1983. PubMed: 6319235

Montrose-Rafizadeh C, et al. High potency antagonists of the pancreatic glucagon-like peptide-1 receptor. J. Biol. Chem. 272: 21201-21206, 1997. PubMed: 9261127

Paul Berg, personal communication

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