pSV2-hph (ATCC® 37647)

Applications: co-transfection marker aminocyclitol phoshotransferase hygromycin B phosphotransferaseexpression vectorshuttle vector  /  Depositors: P Berg, M Dieckmann

Designations pSV2-hph
Permits and Restrictions

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Depositors P Berg, M Dieckmann
Biosafety Level 1
Vector Information
Size (kb): 5.5000000000000000
Vector: pSV2-hph (plasmid)
Promoters: Promoter SV40 early
Construct size (kb): 5.5
Features: marker(s): ampR, hygR
promoter: SV40 early
replicon: pMB1
terminator: SV40
enhancer: SV40 early
co-transfection marker aminocyclitol phoshotransferase hygromycin B phosphotransferase
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): HindIII/BglII--3.87, 1.34, 0.69; PvuII/PstI--3.66, 1.84; PvuII--uncut.
An Escherichia coli/mammalian cell shuttle vector.
Consists of the SV40 promoter (SV nt 270-5171), a HindIII linker, the hph gene (1347 bp), SV40 splice site (SV nt 4710-4100), SV40 polyadenylation site (SV nt 4362-2067),
pML (inserted at the BamHI site with HindIII-EcoRI-amp upstream of the SV40 promoter).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gritz L, Davies J. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179-188, 1983. PubMed: 6319235

Montrose-Rafizadeh C, et al. High potency antagonists of the pancreatic glucagon-like peptide-1 receptor. J. Biol. Chem. 272: 21201-21206, 1997. PubMed: 9261127

Paul Berg, personal communication

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