pSC101 (ATCC® 37032)

Applications: vector with low copy number  /  Depositors: SN Cohen

Permits and Restrictions

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Designations pSC101
Depositors SN Cohen
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 9.263
Vector: pSC101 (plasmid)
Construction: natural isolate
Marker(s):tetR
Construct size (kb): 9.263
Features: marker(s): tetR
replicon: pSC101
Applications
vector with low copy number
Comments
General purpose plasmid vector.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110

Cohen SN, Change AC. Revised interpretation of the origin of the pSC101 plasmid. J. Bacteriol. 132: 734-737, 1977. PubMed: 334752

Bernardi A, Bernardi F. Complete sequence of pSC101. Nucleic Acids Res. 12: 9415-9426, 1984. PubMed: 6096829

Shipped frozen