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Thermus thermophilus (Oshima and Imahori) Williams et al. (ATCC® 27634)

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Strain Designations: HB8  /  Type Strain: yes  /  Biosafety Level: 1

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Deposited As Thermus thermophilus Oshima and Imahori
Verified By Whole-genome Sequencing
Strain Designations HB8
Produces D-xylose isomerase
Produces fructose-1,6-bisphosphatase
Produces phosphofructokinase
Produces phosphoglucomutase
Produces restriction endonuclease TtHB8I
Produces thermostable D-xylose isomerase
Hot spring, Japan
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genome Sequenced Strain

Thermus thermophilus HB8 genomic DNA, complete genome.

Type Strain yes
Heat stability of tRNA and ribosomes
Respiratory proteins
Iron-sulfur protein
Resistance to heavy metal ions
Genome sequenced strain
Medium ATCC® Medium 697: Thermus medium
Growth Conditions
Temperature: 70°C
Atmosphere: Aerobic
Name of Depositor T Oshima
Chain of Custody ATCC <-- T Oshima
Hot spring, Japan

Chiong M, et al. Resistance of Thermus spp. to potassium tellurite. Appl. Environ. Microbiol. 54: 610-612, 1988.

Lehmacher A, Bisswanger H. Isolation and characterization of an extremely thermostable D-xylose isomerase from Thermus aquaticus HB 8. J. Gen. Microbiol. 136: 679-686, 1990.

Yoshida M, et al. The thermostable allosteric enzyme: phosphofructokinase from an extreme thermophile. Biochem. Biophys. Res. Commun. 43: 36-39, 1971. PubMed: 4252961

Yoshida M, Oshima T. The thermostable allosteric nature of fructose 1,6-diphosphatase from an extreme thermophile. Biochem. Biophys. Res. Commun. 45: 495-500, 1971. PubMed: 4334342

Sato S, et al. A DNA methylase from Thermus thermophilus HB8. J. Biochem. 88: 737-747, 1980. PubMed: 6448253

. . J. Gen. Appl. Microbiol. 17: 513-517, 1971.

Degryse E, et al. A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch. Microbiol. 117: 189-196, 1978. PubMed: 678024

Oshima T, Imahori K. Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int. J. Syst. Bacteriol. 24: 102-112, 1974.

Williams RA, et al. DNA relatedness of Thermus strains, description of Thermus brockianus sp. nov., and proposal to reestablish Thermus thermophilus (Oshima and Imahori) [published erratum appears in Int. J. Syst. Bacteriol. 46: 629, 1996]. Int. J. Syst. Bacteriol. 45: 495-499, 1995. PubMed: 8590676

Venegas A, et al. A rapid procedure for purifying a restriction endonuclease from Thermus thermophilus (Tth I). FEBS Lett. 109: 156-158, 1980. PubMed: 6243575

Yoshida T, et al. Respiratory proteins from the extremely thermophilic aerobic bacterium, Thermus thermophilus. Purification procedures for cytochromes c552, c555,549, and c1aa3 and chemical evidence for a single subunit cytochrome aa3. J. Biol. Chem. 259: 112-123, 1984. PubMed: 6323398

Fee JA, et al. Purification and characterization of the Rieske iron-sulfur protein from Thermus thermophilus. Evidence for a [2Fe-2S] cluster having non- cysteine ligands. J. Biol. Chem. 259: 124-133, 1984. PubMed: 6323399

Yoshizaki F, et al. Studies on phosphoglucomutase from an extreme thermophile, Flavobacterium thermophilum HB8. I. Thermostability and other enzymatic properties. J. Biochem. 69: 1083-1089, 1971. PubMed: 5091939

Sato S, Shinomiya T. An isochizomer of TaqI from Thermus thermophilus HB8. J. Biochem. 84: 1319-1321, 1978. PubMed: 730757

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

hot spring

Bergey's Manual of Systematic Bacteriology, Vol. 1

Cross References

Nucleotide (GenBank) : M35674 T.thermophilus 23S ribosomal RNA gene.

Nucleotide (GenBank) : X58342 T.thermophilus 16S ribosomal RNA, part.

Nucleotide (GenBank) : M33159 T.thermophilus insertion sequences Is1000A and Is1000B.

Nucleotide (GenBank) : X06657 Thermus thermophilus HB8 tuf gene for elongation factor Tu (EF-Tu).

Nucleotide (GenBank) : U17352 Thermus aquaticus L-glutamine:D-fructose-6-P amidotransferase (glmS) gene, complete cds and s-layer P100 (slp) gene, partial cds.

Nucleotide (GenBank) : AP008226 Thermus thermophilus HB8 genomic DNA, complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019