SNU-16 ATCC ® CRL-5974™ Homo sapiens stomach; derived from

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Organism	Homo sapiens, human
Tissue	stomach; derived from metastatic site: ascites
Product Format	frozen
Morphology	epithelial
Culture Properties	suspension, multicell aggregates
Biosafety Level	1
Disease	gastric carcinoma
Age	33 years
Gender	female
Ethnicity	Asian
Storage Conditions	liquid nitrogen vapor phase

Karyotype	This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.
Derivation	SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy.
Clinical Data	33 years Asian female SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy.
Antigen Expression	Blood Type A; Rh + The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
Receptor Expression	vasoactive intestinal peptide (VIP), expressed RefPark JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397
Oncogene	myc +; erb B2 +
Genes Expressed	myc +; erb B2 +,Blood Type A; Rh +,The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
Comments	SNU-16 cells were positive for VIP receptors but lacked gastrin receptors. The line was established from cells taken prior to chemotherapy. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C

STR Profile	Amelogenin: X CSF1PO: 12 D13S317: 8,12 D16S539: 11,13 D5S818: 10,13 D7S820: 12 THO1: 6,9 TPOX: 11 vWA: 16
Population Doubling Time	27 hrs

Name of Depositor	J Park
Year of Origin	1987
References	Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Name of Depositor

J Park

Year of Origin

1987

References

Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cancer cell line mutation data
References	Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

SNU-16 (ATCC® CRL-5974™)

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SNU-16 (ATCC^® CRL-5974^™)