SNU-16 (ATCC® CRL-5974)

Organism: Homo sapiens, human  /  Tissue: stomach; derived from metastatic site: ascites  /  Disease: gastric carcinoma

Organism Homo sapiens, human
Tissue stomach; derived from metastatic site: ascites
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Disease gastric carcinoma
Age 33 years
Gender female
Ethnicity Asian
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a human cell line with the bimodal chromosome number distributions. Both modal populations were hypotetraploid. Cells with higher ploidies occurred at 1%. Nine marker chromosomes were common to all cells: t(4q21q); der(5)t(2;5) (q11.2;q13); i(5p); del(17) (p11.2); del(10) (q22.1) and four others. Of these, del(17) generally had two copies per cell. Seven or more other marker chromosomes including HSR(11) (p15.1) were found in some cells only. Multiple copies of DMs were present in most cells. Consistently there were three normal X chromosomes per cell. N7 had six copies and N14, five copies per cell. Normal N12 was not found.
Derivation
SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The line was established from cells taken prior to chemotherapy. 
Clinical Data
33 years
Asian
female
SNU-16 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach.
The line was established from cells taken prior to chemotherapy.
Antigen Expression
Blood Type A; Rh +
The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
Receptor Expression
vasoactive intestinal peptide (VIP), expressed
Oncogene myc +; erb B2 +
Genes Expressed
myc +; erb B2 +,Blood Type A; Rh +,The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
Effects
Yes, the cells have a reported colony forming efficiency of 10% in semisolid medium.
Comments
SNU-16 cells were positive for VIP receptors but lacked gastrin receptors.
The line was established from cells taken prior to chemotherapy.
No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The c-myc proto-oncogene was apmplified, but expressed c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.
The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 8,12
D16S539: 11,13
D5S818: 10,13
D7S820: 12
THO1: 6,9
TPOX: 11
vWA: 16
Population Doubling Time 27 hrs
Name of Depositor J Park
Year of Origin 1987
References

Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Basic Documentation
Other Documentation
References

Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.