SU-DHL-10 (ATCC® CRL-2963)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: lymph node
Derived from Metastatic Site: peritoneal effusion

 /  Disease: Large Cell Lymphoma

Organism Homo sapiens, human
Tissue lymph node
Derived from Metastatic Site: peritoneal effusion

Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast-like
Culture Properties suspension, multicellular aggregates
Biosafety Level 1
Disease Large Cell Lymphoma
Age 25 years
Gender Male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images CRL-2963 Micrograph
Clinical Data 25 years
Caucasian
Male
Comments ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 104 to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 106 and 2.0 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended.
Medium renewal: every 2 to 4 days
Cryopreservation Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 11, 12
D13S317: 10, 11
D7S820: 8, 11
D16S539: 10, 11
vWA: 17
THO1: 8, 9.3
TPOX: 8
CSF1PO: 10, 13
Amelogenin: XY

Isotype IgG lambda
Name of Depositor A Epstein
Year of Origin 1978