SU-DHL-5 (ATCC® CRL-2958)

Organism: Homo sapiens, human  /  Tissue: Organ: lymph node
Derived from Metastatic Site: peritoneal effusion
 /  Cell Type: B lymphocyte

Organism Homo sapiens, human
Tissue Organ: lymph node
Derived from Metastatic Site: peritoneal effusion
Cell Type B lymphocyte
Morphology lymphoblast-like
Culture Properties suspension, multicell aggregates and single cells
Biosafety Level 1
Age 17 years
Storage Conditions liquid nitrogen vapor phase
Karyotype Human hyperdiploid karyotype with 1.5% polyploidy - 47(41-48)<2n>XX, +12, del(6)(q13), del(12)(q13) - sideline with del(6)x2
Clinical Data 17 years
Female
Caucasian
Markers

Cytochemical Analysis:
AcP++ (acid phosphatase)
NASDA++( Naphtol AS-D acetate esterase)
NASDAF++Naphtol AS-D acetate esterase with NaF inhibition)
PAS+eriodic Acid Schiff reaction)
NA+(alpha-naphthyl acetate esterase)
Oil Red O+
PAScD-(Periodic Acid Schiff reaction with diastase digestion)
SBB-(Sudan Black B)
Peroxidase-
AcPcT-acid phosphatase with tartrate)
Alk P-(alkaline phosphatase)
NCA- ( Naphtol AS-D chloroacetate esterase)
gl-(beta-glucuronidase)
LAP-( Leucocyte alkaline phosphatase)

Antigen Expression:

CD3-, CD10+, CD13-, CD19+, CD20+, CD34-, CD37+, CD38-, cyCD79a+, CD80-, HLA-DR +, sm/cyIg-, sm/cyIgM+, sm/cykappa-, sm/cylambda+

Surface Receptors: Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed)
Genes Expressed Fc (not expressed),   IgMEAC  (not expressed), IgGEA (not expressed), E  (not expressed)
Comments This cell line is negative for Candida ingestion.
The depositor states that this cell line is negative for Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Protocol:Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 104 to 1 X 105 viable cells/ mL. Maintain cultures at a cell concentration below 5 X 105 viable cells/ mL.
Medium renewal: Two to three times weekly
Culture Conditions

Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%

STR Profile
D5S818: 12, 15
D13S317: 12
D7S820: 10
D16S539: 11, 13
vWA: 17
TH01: 6, 9
Amelogenin: X
TPOX: 8
CSF1PO: 11, 13
Year of Origin 1978
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Basic Documentation
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794