SU-DHL-5 (ATCC® CRL-2958)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: lymph node; derived from metastatic site: peritoneal effusion  /  Disease: large cell lymphoma; diffuse mixed histiocytic and lymphoma

Organism Homo sapiens, human
Tissue lymph node; derived from metastatic site: peritoneal effusion
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast-like
Culture Properties suspension, multicell aggregates and single cells
Biosafety Level 1
Disease large cell lymphoma; diffuse mixed histiocytic and lymphoma
Gender Female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Human hyperdiploid karyotype with 1.5% polyploidy - 47(41-48)<2n>XX, +12, del(6)(q13), del(12)(q13) - sideline with del(6)x2
Images CRL-2958 Micrograph
Clinical Data 17 years
Female
Caucasian
Antigen Expression CD3-, CD10+, CD13-, CD19+, CD20+, CD34-, CD37+, CD38-, cyCD79a+, CD80-, HLA-DR +, sm/cyIg-, sm/cyIgM+, sm/cykappa-, sm/cylamb

Receptor Expression Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed)
Comments The depositor states that this cell line is negative for Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
This cell line is negative for Candida ingestion.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 104 to 1 X 105 viable cells/ mL. Maintain cultures at a cell concentration below 5 X 105 viable cells/ mL.
Medium renewal: Two to three times weekly
Cryopreservation Storage temperature: liquid nitrogen vapor phase
Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Growth Conditions: Culture can be maintained by the addition of fresh medium or re-seeding a new flask with cells in culture.
STR Profile
D5S818: 12, 15
D13S317: 12
D7S820: 10
D16S539: 11, 13
vWA: 17
TH01: 6, 9
Amelogenin: X
TPOX: 8
CSF1PO: 11, 13
Name of Depositor A Epstein
Year of Origin 1978