GA-10 (ATCC® CRL-2392)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: Burkitt's lymphoma

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Burkitt's lymphoma
Age 41 years
Gender male
Ethnicity Caucasian, White
Storage Conditions liquid nitrogen vapor phase
Karyotype The reported karyoype was 46, XY, dup(1) (pter to q32::q21 to qter), t(8;14) (q24.1; q32), -10, +der(10)t(Y;10)(q12:q26), -21, +der (21) t (?; 21) (?; p11)., In addition a few cells lacked a second chromosome 21 and carried an additional der(21) t(?;21) (?;q22). The Vh segment in this cell line was Vh4-34 (Vh 4.21).
Derivation

GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.

The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy).

Clinical Data
41 years
Caucasian
male
Antigen Expression
CD10 +, CD19 +, CD20 +, CD40 + (10%), CD45 +, CD77 + (90%), CD34 -
Genes Expressed
immunoglobulin; kappa light chain
Cellular Products
immunoglobulin; kappa light chain
Comments

The cells express relatively high levels of CD77 (approximately 90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).

GA-10 cells exhibit sensitivity to Shiga Toxin (Stx) in toxicity studies.

This cell line is slightly more sensitive to doxorubicin and etoposide than HL-60 leukemia cells.

Flavopiridol, a potent inhibitor of cell cycle progression through G1 and G2, produces apoptosis of the GA-10 cells at relatively low concentrations of 20-30 nM.

GA-10 cells are negative for Epstein-Barr virus (EBV).

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,20
Population Doubling Time 24 hrs
Name of Depositor AP Rapoport
Year of Origin 1996
References

Rapoport AP, et al. Flavopiridol induces apoptosis and caspase-3 activation of a newly characterized Burkitt's lymphoma cell line containing mutant p53 genes. Blood Cells Mol Dis 27(3):610-24, 2001. PubMed: 11482875

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rapoport AP, et al. Flavopiridol induces apoptosis and caspase-3 activation of a newly characterized Burkitt's lymphoma cell line containing mutant p53 genes. Blood Cells Mol Dis 27(3):610-24, 2001. PubMed: 11482875

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.