MA-104 Clone 1 (ATCC® CRL-2378.1™)

Organism: Cercopithecus aethiops  /  Cell Type: epithelial

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Cercopithecus aethiops
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age embryo
Applications
transfection host
Storage Conditions liquid nitrogen vapor phase
Virus Susceptibility Simian rotavirus
Comments
This item is not available for international distribution.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: 1:3 to 1:10
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Population Doubling Time 27 hours
Name of Depositor MM Vincent
Year of Origin 2001
References

Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev. Biol. Stand. 60: 125-131, 1985. PubMed: 4043530

MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue. Chromosome and isoenzyme analysis of the cells distributed by some sources showed that the species of origin was African green monkey (PubMed: 4043530). In May of 1994, M.M. Vincent deposited ampules of MA-104 at passage 3 at the ATCC. At passage 16 and higher, presence of both African green monkey and Rhesus monkey cells were detected by isoenzyme analysis. An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. (ATCC CRL-2378). This line is available as MA-104 clone 1 (CRL-2378.1).

Roche Transfection Reagents

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev. Biol. Stand. 60: 125-131, 1985. PubMed: 4043530

MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue. Chromosome and isoenzyme analysis of the cells distributed by some sources showed that the species of origin was African green monkey (PubMed: 4043530). In May of 1994, M.M. Vincent deposited ampules of MA-104 at passage 3 at the ATCC. At passage 16 and higher, presence of both African green monkey and Rhesus monkey cells were detected by isoenzyme analysis. An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. (ATCC CRL-2378). This line is available as MA-104 clone 1 (CRL-2378.1).

Roche Transfection Reagents