EM9 (DNA repair mutant of CHO) (ATCC® CRL-1861)

Organism: Cricetulus griseus, hamster, Chinese  / 

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial-like
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Gender female
Applications
EM9 is a repair deficient mutant derived from AA8 (see ATCC CRL-1859).
The line is defective in single strand break repair, has a 10 fold higher baseline frequency of sister chromatid exchange relative to AA8 and a 2 fold greater sensitivity to killing by X-rays.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).
EM9 is a repair deficient mutant derived from AA8 (see ATCC CRL-1859).
Clinical Data
female
Comments
This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).
EM9 is a repair deficient mutant derived from AA8 (see ATCC CRL-1859).
The line was selected for enhanced sensitivity to ethylmethanesulfonate (EMS).
The line is defective in single strand break repair, has a 10 fold higher baseline frequency of sister chromatid exchange relative to AA8 and a 2 fold greater sensitivity to killing by X-rays.
This defect is corrected by the human XRCC1 gene.
Complete Growth Medium Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Subculturing
Protocol:
  1. To subculture attaced cells, remove culture medium.
  2. The suspended cells are viable and can be used to start new cultures.
  3. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  4. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  5. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  6. Add appropriate aliquots of the cell suspension to new culture vessels.
  7. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor LH Thompson
References

Thompson LH, et al. A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange. Mutat. Res. 95: 427-440, 1982. PubMed: 6889677

Thompson LH, et al. A screening method for isolating DNA repair-deficient mutants of CHO cells. Somatic Cell Genet. 6: 391-405, 1980. PubMed: 7404270

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Thompson LH, et al. A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange. Mutat. Res. 95: 427-440, 1982. PubMed: 6889677

Thompson LH, et al. A screening method for isolating DNA repair-deficient mutants of CHO cells. Somatic Cell Genet. 6: 391-405, 1980. PubMed: 7404270