JH4 clone 1 (ATCC® CCL-158)

Organism: Cavia porcellus, guinea pig  /  Tissue: lung  / 

Organism Cavia porcellus, guinea pig
Tissue lung
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype normal female; diploid; stable
Clinical Data
female
Complete Growth Medium Ham's F12K medium, 90%; fetal bovine serum, 10%
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM  EDTA solution to remove all traces of serum which  contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell   layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor R Hay
References

Hoying J. Selection and histochemical identification of epithelial-like cells from guinea pig lung M.Sc. thesis, Wright State Univ., 1975

Cross References

Nucleotide (GenBank) : AF131949 Cavia porcellus tissue factor mRNA, complete cds.

Basic Documentation
References

Hoying J. Selection and histochemical identification of epithelial-like cells from guinea pig lung M.Sc. thesis, Wright State Univ., 1975