Bacillus subtilis subsp. spizizenii Nakamura et al. (ATCC® CRM-6633)

Strain Designations: NRS 231  /  Type Strain: no  /  Biosafety Level: 1

Permits And Restrictions

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Deposited As Bacillus subtilis (Ehrenberg) Cohn
Strain Designations NRS 231
Application For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009
Assay of amoxicillin
Assay of bekanamycin aminodeoxykanamycin, kanamycin B, kanendomycin
Assay of capreomycin sulfate capreomycin, capromycin
Assay of colistimethate sodium
Assay of colistin colimycin
Assay of dactinomycin actinomycin, actinomycin D
Assay of dihydrostreptomycin sulfate dihydrostreptomycin
Assay of erythromycin
Assay of framycetin
Biotechnology
Assay of gentamicins gentamicin
Assay of hygromycin B
Assay of kanamycin
Assay of lactam antibiotics beta-lactam antibiotics
Assay of lasalocid X-537A, lasalocid A
Assay of mitomycin mitomycin C
Assay of monensin
Assay of neomycin
Assay of novobiocin
Assay of penicillin
Assay of polymyxin B
Assay of rifampin rifampicin, rifamycin AMP
Assay of spiramycin
Assay of streptomycin
Assay of streptonigrin
Assay of tobramycin
Assay of vancomycin
Control strain
Media testing
Membrane filter testing
Produces restriction endonuclease Bsu6633I
Quality control strain
Sporicidal test
Sterility testing
Susceptibility disc testing novobiocin
Susceptibility disc testing rifampin rifampicin, rifamycin AMP
Susceptibility testing
Testing antibacterial activity
Testing bactericides
Validation control
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Intended Use

 

 

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

 

    ACLASS Accredited Reference Material Producer AR-1384

Preceptrol® no
Type Strain no
Comments Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

Taxonomy

Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Name of Depositor NR Smith
Special Collection ATCC
References

Waleh NS, Ingraham JL. Pyrimidine ribonucleoside monophosphokinase and the mode of RNA turnover in Bacillus subtilis. Arch. Microbiol. 110: 49-54, 1976. PubMed: 189719

Shibata T, et al. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains. J. Bacteriol. 128: 473-476, 1976. PubMed: 824277

Ikawa S, et al. Genetic studies on site-specific endodeoxyribonucleases in Bacillus subtilis: multiple modification and restriction systems in transformants of Bacillus subtilis 168. Mol. Gen. Genet. 177: 359-368, 1980. PubMed: 6246395

The genus Bacillus, Agriculture Handbook 427. Washington, DC: ARS/USDA; 1973.

British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003

British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 2003

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

. Biotechnology and food safety. Boston: Butterworth-Heinemann; 1990.

AOAC International Monensin in feeds, microbiological method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 972.56.

European Pharmacopoeia Commission Sterility. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.1, 1997

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (total viable aerobic count). Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.12, 1997

European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997

US Food & Drug Administration. GENERAL BIOLOGICAL PRODUCTS STANDARDS; General Provisions; Sterility Code of Federal Regulations Title 21: 21CFR610.12, Subpart B, 2005

AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23.

Noya F, et al. Heme compounds as iron sources for nonpathogenic Rhizobium bacteria. J. Bacteriol. 179: 3076-3078, 1997. PubMed: 9139934

Kiesau P, et al. Evidence for a multimeric subtilin synthetase complex. J. Bacteriol. 179: 1475-1481, 1997. PubMed: 9045802

Yeaman MR, et al. Purification and in vitro activities of rabbit platelet microbicidal proteins. Infect. Immun. 65: 1023-1031, 1997. PubMed: 9038312

Lode H, et al. Comparative clinical pharmacology of three ampicillins and amoxicillin administered orally. J. Infect. Dis. 129: S156-S168, 1974. PubMed: 4834960

Frerichs GN, Chandler MD. The identification and assay of antibiotics in live virus vaccines by electrophoresis in gel. J. Biol. Stand. 10: 205-211, 1982. PubMed: 6813333

Lightbown JW, Dixon H. The first international reference preparation of tobramycin. J. Biol. Stand. 10: 157-168, 1982. PubMed: 7096362

Jansen EF, Hirschmann DJ. Subtilin -- an antibacterial product of Bacillus subtilis. Culturing conditions and properties. Arch. Biochem. 4: 297-309, 1944.

Bowman FW, et al. Collaborative study of aerobic media for sterility testing by membrane filtration. J. Pharm. Sci. 60: 1087-1088, 1971. PubMed: 5000479

Simon HJ, Yin EJ. Microbioassay of antimicrobial agents. Appl. Microbiol. 19: 573-579, 1970. PubMed: 4986725

Pittillo RF, Woolley C. Biological assay of streptonigrin (NSC 45383) in body fluids and tissues of mice. Antimicrob. Agents Chemother. 5: 82-85, 1974. PubMed: 4209070

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

Nakamura LK, et al. Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov.. Int. J. Syst. Bacteriol. 49: 1211-1215, 1999. PubMed: 10425781

Supplemental assay method for testing growth promoting qualities of fluid thioglycollate medium and soybean-casein digest medium using Bacillus Subtilis spores and Candida krusei as the indicator organisms. :US Department of Agriculture;USDA, Center for Veterinary Biologics STSAM0900.01, 1999

Supplemental assay method for testing growth-promoting qualities of brain heart infusion agar using Bacillus subtilis spores and Candida krusei as indicator organisms. :US Department of Agriculture;USDA, Center for Veterinary Biologics STSAM0902.01, 1999

Supplemental assay method for testing for preservative interference with sterility tests. :US Department of Agriculture;USDA, Center for Veterinary Biologics STSAM0903.01, 1999

Microbial assay for antibiotics. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.34.

Microbial limit test. Tokyo, Japan:Japanese Pharmacopoeia;JP Jp14e.part I.35.

Microbial limit test for crude drugs. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.36.

Sterility test, Growth promotion test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.54.

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14698-1:2003.

Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: carrier of agents for agar diffusion test. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-2.

Methods for the determination of susceptibility of pathogenic bacteria (without mycobacteria) to chemotherapeutic agents of discs for agar diffusion test example for getting the standard curve. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-2 Beiblatt 1.

Medical microbiology - Susceptibility testing of pathogens to antimicrobial agents - Part 2: Active substance carriers for the agar diffusion test; Carrier loads and values required for drawing a standard curve. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-2 Beiblatt 2: 2002

Standard Test Method for Confirming the Sterility of Membrane Filters. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4196-05.

Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity. London, UK:British Standards Institution;British Standard BS EN 12353:2006.

Chemical disinfectants --- Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants used in food, industrial, domestic and intitutional areas --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 13704:2002.

Chemical disinfectants and antiseptics --- Surgical hand disinfection --- Test method and requirement (phase 2/step 2). London, UK:British Standards Institution;British Standard BS EN 12791:2005.

Chemical disinfectants and antiseptics --- Basic sporicidal activity --- Test method and requirements (phase 1, step 1). London, UK:British Standards Institution;British Standard BS EN 14347:2005.

Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution;British Standard BS EN 14885:2006.

Cleanrooms and associated controlled environments --- Biocontamination control --- Part 1: General principles and methods. London, UK:British Standards Institution;British Standard BS EN ISO 14698:2003.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

Cross References

Nucleotide (GenBank) : M38634 B.subitilis cellulase gene, 5' end, clone pBAG10.

Nucleotide (GenBank) : J03767 B.subtilin spaS gene encoding subtilin, complete cds.

Nucleotide (GenBank) : L07785 Bacillus subtilis spaR and spaK genes, complete coding regions.

Nucleotide (GenBank) : M83944 Bacillus subtilis subtilin (spaD, spaB, spaC, spaS) genes, complete cds.

Nucleotide (GenBank) : AB018486 Bacillus subtilis gene for 16S rRNA, partial sequence, strain:ATCC 6633.

Nucleotide (GenBank) : AF004102 Bacillus subtilis strain ATCC 6633 thymidylate synthase (thyA) gene, partial cds.

Nucleotide (GenBank) : J03294 Bacillus subtilis thioredoxin (trx), uvrB and aspartokinase II genes, complete cds.

Nucleotide (GenBank) : L24075 Bacillus subtilis (ATCC 6633) spaB gene involved in subtilin biosynthesis, complete cds.

Nucleotide (GenBank) : M99263 Bacillus subtilis operon encoding spaB, spaC, spaD, spaE and spaS genes, complete cds's; ORF, complete cds.

Nucleotide (GenBank) : U38418 Bacillus subtilis chimeric proU operon, proV, proW, proX and proZ genes, complete cds, and spaE gene, partial cds.

Nucleotide (GenBank) : U09819 Bacillus subtilis spaB, spaT, spaC, subtilin (spaS), spaI, spaF, spaE, spaG, spaR spaK and YvaN genes, complete cds.

Nucleotide (GenBank) : M86869 Bacillus subtilis spaB gene, 3' end; spaT gene, complete cds; spaC gene, complete cds; subtilin (spaS) gene, putative cds.

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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