J.RT3-T3.5 (ATCC® TIB-153)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Tissue: peripheral blood  /  Disease: acute T cell leukemia

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute T cell leukemia
Gender male
Applications
transfection host
Storage Conditions liquid nitrogen vapor phase
Derivation
The J.RT3-T3.5 cell line is a derivative mutant of the Jurkat leukemia cell line.
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM.
The line was produced by treatment with ethylmethanesulfonate and negative selection with OKT3 monoclonal antibody.
This is a mutant line derived from the E6-1 clone of Jurkat (ATCC TIB 152) that lacks the beta chain of the T cell antigen receptor.
Clinical Data
male
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al.
Comments

 J.RT3-T3.5 cells lack the beta chain of the T cell antigen receptor.

The cells do not express either CD3 or the T cell receptor alpha/beta heterodimer on the surface.

The cells may be useful for transfection studies involving the T cell receptor beta chain gene.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor A Weiss
References

Ohashi PS, et al. Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. Nature 316: 606-609, 1985. PubMed: 4033759

Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306

Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013

Ronald Wange, personal communication

Basic Documentation
References

Ohashi PS, et al. Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer. Nature 316: 606-609, 1985. PubMed: 4033759

Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306

Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013

Ronald Wange, personal communication