UC10-4F10-11 (ATCC® HB-304)

Organism: hamster (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism hamster (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a.
The antibody can be used to detect and quantitate CTLA-4 expression by flow cytometric analysis, and can be used to immunoprecipitate the antigen from CTLA-4 positive cells.
Under conditions of suboptimal T cell receptor (TCR) stimulation, whole antibody or Fab fragments from 4F10 can augment T cell proliferation in allogeneic mixed lymphocyte reactions.
However, when optimal TCR stimulation is present, 4F10 can suppress T cell proliferation.
Derivation
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CTLA-4
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CTLA-4
Comments
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a.
The antibody can be used to detect and quantitate CTLA-4 expression by flow cytometric analysis, and can be used to immunoprecipitate the antigen from CTLA-4 positive cells.
Under conditions of suboptimal T cell receptor (TCR) stimulation, whole antibody or Fab fragments from 4F10 can augment T cell proliferation in allogeneic mixed lymphocyte reactions.
However, when optimal TCR stimulation is present, 4F10 can suppress T cell proliferation.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG
Name of Depositor JA Bluestone
References

Walunas TL, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1: 405-413, 1994. PubMed: 7882171

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Walunas TL, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1: 405-413, 1994. PubMed: 7882171