ATRFLOX [Mutatect] (ATCC® CRL-2780™)

Organism: Homo sapiens, human  /  Cell Type: epithelial

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease colorectal carcinoma
Age adult
Gender male
Applications
molecular studies ataxia telangiectasia and Rad3 related FRAP-related protein-1, Rad3 related protein, protein kinase ATR
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
This cell line is a derivative of ATCC Catalog No. CCL-247 (HCT 116) in which one copy of the ATR (ataxia telangiectasia related) gene has been disrupted, and the other allele has been fixed with lox sites flanking exon 2 making it susceptible to Cre deletion. [PubMed: 11721054].
Clinical Data
male
Genes Expressed
ataxia telangiectasia and Rad3 related
Comments
This cell line is a derivative of ATCC Catalog No. CCL-247 (HCT 116) in which one copy of the ATR (ataxia telangiectasia related) gene has been disrupted, and the other allele has been fixed with lox sites flanking exon 2 making it susceptible to Cre deletion. [PubMed: 11721054].
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 7,9
D13S317: 10,12
D16S539: 11,13
D5S818: 10,12
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22
Name of Depositor SJ Elledge
References

This cell line is a derivative of HCT 116 in which one copy of the ATR gene has been disrupted, and the other allele has been fixed with lox sites flanking exon 2 making it susceptible to Cre deletion.

Cortez D, et al. ATR and ATRIP: partners in checkpoint signaling. Science 294: 1713-1716, 2001. PubMed: 11721054

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cell Micrograph
References

This cell line is a derivative of HCT 116 in which one copy of the ATR gene has been disrupted, and the other allele has been fixed with lox sites flanking exon 2 making it susceptible to Cre deletion.

Cortez D, et al. ATR and ATRIP: partners in checkpoint signaling. Science 294: 1713-1716, 2001. PubMed: 11721054