A frozen ampule at population doubling 2.3 was received at the ATCC in April, 2000.
Yi X, et al. Both transcriptional and posttranscriptional mechanisms regulate human telomerase template RNA levels. Mol. Cell. Biol. 19(6): 3989-3997, 1999. PubMed: 10330139
Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332
Huffman KE, et al. Telomere shortening is proportional to the size of the G-rich telomeric 3'-overhang. J. Biol. Chem. 275: 19719-19722, 2000. PubMed: 10787419
Morales CP, et al. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase. Nat. Genet. 21: 115-118, 1999. PubMed: 9916803
These cells have been reported to have a maximal life span of 85 to 90 population doublings.