GA-10 (Clone 4) (ATCC® CRL-2393)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: Burkitt's lymphoma

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Burkitt's lymphoma
Age 41 years
Gender male
Ethnicity Caucasian, White
Storage Conditions liquid nitrogen vapor phase
Derivation
GA-10 (Clone 4) is a subclone of the GA-10 (ATCC CRL-2392) cell line derived by culturing the parental line on a feeder layer.

GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.


 

Antigen Expression
CD77 + (90%)
Comments The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy). 

This clone of cells also appears to express high levels of CD77 (approximately 90%); CD77 is also known as Burkitt's lymphoma antigen (BLA). 

GA-10 (Clone-4) cells exhibit sensitivity to Shiga Toxin (Stx) in toxicity studies.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete culture medium, 95%, described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,20
Name of Depositor AP Rapoport
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation