BC-2 (ATCC® CRL-2231)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: lymphoma

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells contain EBV and KSHV (HHV-8) viral genomes
Disease lymphoma
Age 31 years adult
Gender male
Ethnicity Caucasian
Applications
transfection host
Derivation
BC-2 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles.
The cells were derived from a pleural cavity based lymphomatous effusion.
Clinical Data
male
Caucasian
31 years adult
Antigen Expression
CD45 +; EMA + (epithelial membrane antigen)
Genes Expressed
CD45 +; EMA + (epithelial membrane antigen)
Comments
BC-2 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles.
The cells were derived from a pleural cavity based lymphomatous effusion.
The cells contain two viral genomes: Epstein-Barr virus (EBV) and Kaposi sarcoma associated herpesvirus (KSHV, provisionally designed HHV-8).
KSHV is a recently identified and largely uncharacterized virus.
The BC-2 cell line allows in vitro culture of KSHV viral genomes.
It is also a practical source of KSHV DNA to be used as a positive control for screening tests.
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Population Doubling Time 48 to 72 hrs
Name of Depositor Cornell Res. Fndn., Inc.
References

Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568

Cesarman E, et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86: 2708-2714, 1995. PubMed: 7670109

Chadburn A, et al. CD30 (Ki-1) positive anaplastic large cell lymphomas in individuals infected with the human immunodeficiency virus. Cancer 72: 3078-3090, 1993. PubMed: 8221575

Cesarman E, et al. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS- related body-cavity-based lymphomas. N. Engl. J. Med. 332: 1186-1191, 1995. PubMed: 7700311

Cesarman E, et al. KSHV positive cell lines. US Patent 5,908,773 dated Jun 1 1999

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568

Cesarman E, et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86: 2708-2714, 1995. PubMed: 7670109

Chadburn A, et al. CD30 (Ki-1) positive anaplastic large cell lymphomas in individuals infected with the human immunodeficiency virus. Cancer 72: 3078-3090, 1993. PubMed: 8221575

Cesarman E, et al. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS- related body-cavity-based lymphomas. N. Engl. J. Med. 332: 1186-1191, 1995. PubMed: 7700311

Cesarman E, et al. KSHV positive cell lines. US Patent 5,908,773 dated Jun 1 1999