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Karyotype
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modal number = 77, YY; seven markers involving chromosomes 1, 7, 14, X, and 18 were recognized. The other chromosomes involved could not be properly identified.; Monosomy 7 and monosomy 14 were present in all 15 metaphases analyzed. No normal chromosomes 5 or 18 were observed.
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Derivation
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LS1034 is a colorectal carcinoma cell line isolated in 1989 from a primary tumor biopsy from a 54-year old Caucasian male patient diagnosed with Dukes' Type C, moderately to poorly differentiated cecal carcinoma.
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Antigen Expression
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carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive
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Oncogene
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p53 + (mutated, Gly --> Ser mutation at position 245), APC (mutated, deletion, GAAAAGATT --> GATT at codon 1309)
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Genes Expressed
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p53 + (mutated, Gly --> Ser mutation at position 245), APC (mutated, deletion, GAAAAGATT --> GATT at codon 1309),carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive
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Comments
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More than 90% of LS1034 cells express surface CEA.
The cells express the major histocompatibility (MHC) class I antigens and beta 2 microglobulin, but class II antigens, (HLA-DR, DQ, and DP) were not detected.
No measurable amount of latent transforming growth factor beta-1 (TGF beta-1) is secreted.
Picomolar concentrations of TGF beta-1, TGF beta-2, and TGF beta-3 inhibit the proliferation of LS1034 cells.
LS1034 cells are multidrug resistant (MDR), and can proliferate in serum free medium.
The patient did not exhibit familial adenomatous polyposis (FAP) and normal tissue did not have the deletion in the APC gene.
The colony forming efficiency was 3% in methylcellulose medium containing 5% fetal bovine serum, and the clonogenicity is increased 600% in serum free methylcellulose as compared to semisolid medium containing serum.
A culture submitted to the ATCC in September 1994 was found to be contaminated with mycoplasma, and was cured by a 21 day treatment with BM Cycline.
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