PFSK-1 (ATCC® CRL-2060™)

Organism: Homo sapiens, human  / 

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease malignant primitive neuroectodermal tumor
Age 22 months
Gender male
Ethnicity Caucasian
Applications
PFSK-1 cells form colonies in soft agar, and lack contact inhibition.
They express the intermediate filament protein, nestin, and are positive for neuron specific enolase (NSE).
They lack characterisics of terminally differentiated neurons or glia.
Restriction fragment length polymorphism studies showed loss of heterozygosity for multiple loci on chromosome 17.
Neither c-myc nor N-myc is amplified or re-arranged.
Storage Conditions liquid nitrogen vapor temperature
Karyotype hypotetraploid; 84, XXY; -Y, t(Xp;8q), del(1)(p22), -3, del(4)(p14), -9, -10, -13, -14, -14, -16, -22
Clinical Data
male
Caucasian
22 months
Tumorigenic Yes
Comments
PFSK-1 cells form colonies in soft agar, and lack contact inhibition.
They express the intermediate filament protein, nestin, and are positive for neuron specific enolase (NSE).
They lack characterisics of terminally differentiated neurons or glia.
Restriction fragment length polymorphism studies showed loss of heterozygosity for multiple loci on chromosome 17.
Neither c-myc nor N-myc is amplified or re-arranged.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution. Remove the trypsin solution, add fresh trypsin - EDTA (1 to 2 ml), and let the culture sit at room temperature (or at 37C) until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 12,14
D16S539: 13
D5S818: 11,12
D7S820: 10
THO1: 9,9.3
TPOX: 8,9
vWA: 17,18
Population Doubling Time 30 hrs
Name of Depositor D Fults
References

Fults D, et al. Establishment and characterization of a human primitive neuroectodermal tumor cell line from the cerebral hemisphere. J. Neuropathol. Exp. Neurol. 51: 272-280, 1992. PubMed: 1316433

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer Cell Line Mutation Data
References

Fults D, et al. Establishment and characterization of a human primitive neuroectodermal tumor cell line from the cerebral hemisphere. J. Neuropathol. Exp. Neurol. 51: 272-280, 1992. PubMed: 1316433