EJ-6-2-Bam-6a (ATCC® CRL-1888)

Organism: Mus musculus, mouse  /  Disease: Carcinoma

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Carcinoma
Age embryo
Strain NIH/Swiss
Applications
This line was established by C. Shih et al.
Derivation
This line was established by C. Shih et al. in 1981 by transfecting NIH/3T3 cells with Bam H1 digested DNA from EJ-6-2 cells (NIH/3T3 cells transformed with DNA from the human EJ bladder carcinoma EJ).
Tumorigenic Yes
Effects
Yes, forms subcutaneous tumors in BALB/c and NSF mice
Comments
This line was established by C. Shih et al. in 1981 by transfecting NIH/3T3 cells with Bam H1 digested DNA from EJ-6-2 cells (NIH/3T3 cells transformed with DNA from the human EJ bladder carcinoma EJ).
The cells are morphologically transformed and highly tumorigenic in BALB/c and NSF mice.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly.
Name of Depositor RA Weinberg
Year of Origin 1981
References

Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109

Shih C, et al. Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature 290: 261-264, 1981. PubMed: 7207618

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109

Shih C, et al. Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature 290: 261-264, 1981. PubMed: 7207618